Dados do Trabalho

Título

Lycopene supplementation reduces inflammatory, histopathological and DNA damages in an acute lung injury rabbit model

Objetivo

This study aimed to investigate lycopene supplementation on pulmonary oxidative damage in an experimental induced acute lung injury (ALI) model.

Métodos

Fifty-five rabbits were supplemented with 10mg/ kg of lycopene for 21 days prior to the experiment. Lung injury was induced by tracheal infusion of warm saline. CG submitted to Protective CMV, without supplementation (CG; n = 5), ALI animals submitted to MV with (MVL; n = 10) and without lycopene supplementation (MV; n = 10), ALI animals submitted to HFOV with (HFL; n = 10) and without supplementation (HF; n = 10), respectively. Lung oxidative stress and inflammatory response were assessed by number of polymorphonuclear leukocytes in bronchoalveolar lavage fluid, DNA damage and pulmonary histological damage.

Resultados

After 4 hours, HFOV groups and protective CMV with lycopene supplementation, showed a significant oxygenation improvement compared to Protective CMV group without supplementation. MVL and HFL groups presented significantly lower inflammation comparing to animals without supplementation, as well as lower histological injury. DNA damage on lymphocytes comparing animals submitted to protective CMV was significantly lower in animals supplemented with lycopene.

Conclusão

This study demonstrates that lycopene supplementation improved oxygenation, reduced inflammatory injury and DNA damage, as well as histopathological injury score in this model. Both HFOV and animals submitted to protective CMV supplemented with lycopene showed reduced DNA-free damage compared to animals without supplementation.